No current super-resolution technique is yet usable for large scale testing of multiple samples. We propose to solve this problem by adapting a recent microscopy innovation termed expansion microscopy. This technology is based on conventional immunostaining of the samples, followed by embedding in a plastic structure, which is physically expanded by approximately 4.5-fold in the work published so far, allowing an imaging resolution of at least 70 nm. Our laboratory has recently surpassed this, and has optimized an expansion of approximately 10.5-fold, thus providing an imaging resolution of below 30 nm.

Silvio Rizzoli

Principal Investigator
Weitere Teilprojekte

Z2: “Integrative data analysis and interpretation, and generation of a synaptic integrative data strategy (SynIDs)”

Stefan Bonn

A2: “Structure of synaptic organelles by X-ray diffraction and imaging”

Tim Salditt

A6: “Mitochondria function and turnover in synapses”

Peter Rehling