Here we planned to systematically investigate the impact of protein and lipid composition, curvature and lateral membrane tension on domain formation in multicomponent membranes derived from hippocampal neurons and from synaptosomes. We mapped the position of certain proteins found in the membrane with a non-invasive and label-free rapid rupture event imaging, force distance-based atomic force microscopy (AFM). The technologies employed, unfortunately, were not precise enough for a thorough characterization.

Andreas Janshoff

Principal Investigator
More subprojects

B9: “Cytoskeletal alterations contributing to synapto-axonal dysfunction in Parkinson’s disease”

Paul Lingor

Z3: “Simple multi-color super-resolution imaging by 10x expansion microscopy”

Silvio Rizzoli

B1: “The distribution of structural lipids in synaptic membranes”

Nhu Phan