The lipid composition of the synapse has been difficult to study. Structural lipids, including abundant phospholipids, cholesterol or sphingolipids, are poorly understood. Their concentrations in synaptic membranes are unclear, and their distributions are virtually unknown. I propose to approach this problem by combining two state-of-the-art lipid analysis tools, based on secondary ion mass spectrometry (SIMS): time-of-flight SIMS (ToF-SIMS), and nanoscale SIMS (NanoSIMS).

Nhu Phan

Principal Investigator
More subprojects

Z3: “Simple multi-color super-resolution imaging by 10x expansion microscopy”

Silvio Rizzoli

B3: “Mapping the protein and lipid organization in the plasma membrane of neurons using rapid rupture event imaging”

Andreas Janshoff

A6: “Mitochondria function and turnover in synapses”

Peter Rehling