The lipid composition of the synapse has been difficult to study. Structural lipids, including abundant phospholipids, cholesterol or sphingolipids, are poorly understood. Their concentrations in synaptic membranes are unclear, and their distributions are virtually unknown. I propose to approach this problem by combining two state-of-the-art lipid analysis tools, based on secondary ion mass spectrometry (SIMS): time-of-flight SIMS (ToF-SIMS), and nanoscale SIMS (NanoSIMS).

Nhu Phan

Principal Investigator
More subprojects

B8: “Deciphering the major alpha-synuclein-related alterations in the Parkinson’s disease synapse”

Tiago Outeiro

B4: “In vitro reconstitution of inhibitory GABAergic postsynapses”

Claudia Steinem

Associated project: “Modeling of short-term plasticity of nerve-evoked EPSCs at a glutamatergic synapse”

Erwin Neher