The lipid composition of the synapse has been difficult to study. Structural lipids, including abundant phospholipids, cholesterol or sphingolipids, are poorly understood. Their concentrations in synaptic membranes are unclear, and their distributions are virtually unknown. I propose to approach this problem by combining two state-of-the-art lipid analysis tools, based on secondary ion mass spectrometry (SIMS): time-of-flight SIMS (ToF-SIMS), and nanoscale SIMS (NanoSIMS).

Nhu Phan

Principal Investigator
More subprojects

Associated project: “Modeling of short-term plasticity of nerve-evoked EPSCs at a glutamatergic synapse”

Erwin Neher

B2: “A microfluidic in vitro approach to study protein mobility in the synapse”

Sarah Köster/Silvio Rizzoli

B4: “In vitro reconstitution of inhibitory GABAergic postsynapses”

Claudia Steinem