While the protein-protein interactions governing synaptic transmission are well characterized, less is known about how these steps are regulated. Here we will focus on protein phosphorylation and dephosphorylation as a key regulatory mechanism for controlling presynaptic performance. Using MS-based phosphoproteomics, we ask here which proteins change their phosphorylation status during triggering of exocytosis. As model system we plan to use isolated nerve terminals (synaptosomes) that retain calcium-dependent exocytosis, but are accessible to biochemical analysis.

Reinhard Jahn

Principal Investigator

Henning Urlaub

Principal Investigator
More subprojects

Associated project: “Modeling of short-term plasticity of nerve-evoked EPSCs at a glutamatergic synapse”

Erwin Neher

C1: “Theoretical model of activity-dependent AMPA-receptor dynamics in the synapse”

Christian Tetzlaff

B3: “Mapping the protein and lipid organization in the plasma membrane of neurons using rapid rupture event imaging”

Andreas Janshoff