While the protein-protein interactions governing synaptic transmission are well characterized, less is known about how these steps are regulated. Here we will focus on protein phosphorylation and dephosphorylation as a key regulatory mechanism for controlling presynaptic performance. Using MS-based phosphoproteomics, we ask here which proteins change their phosphorylation status during triggering of exocytosis. As model system we plan to use isolated nerve terminals (synaptosomes) that retain calcium-dependent exocytosis, but are accessible to biochemical analysis.

Reinhard Jahn

Principal Investigator

Henning Urlaub

Principal Investigator
More subprojects

B4: “In vitro reconstitution of inhibitory GABAergic postsynapses”

Claudia Steinem

C2: “Dual-purpose optimization theory for the synaptic vesicle cycle”

Fred Wolf

A2: “Structure of synaptic organelles by X-ray diffraction and imaging”

Tim Salditt