Elisa D’Este/Stefan Hell
While the protein-protein interactions governing synaptic transmission are well characterized, less is known about how these steps are regulated. Here we will focus on protein phosphorylation and dephosphorylation as a key regulatory mechanism for controlling presynaptic performance. Using MS-based phosphoproteomics, we ask here which proteins change their phosphorylation status during triggering of exocytosis. As model system we plan to use isolated nerve terminals (synaptosomes) that retain calcium-dependent exocytosis, but are accessible to biochemical analysis.