State-of-the-art mass spectrometry (MS) makes possible the identification and quantification of cellular proteins, including the detailed analysis of post-translational modifications. MS has recently been combined with chemical cross-linking of proteins, with the purpose of identifying protein–protein contact sites. I now plan to extend cross-linking mass spectrometry to the investigation of protein interactions from native material. I will investigate isolated nerve terminals (synaptosomes) in resting and stimulated (K+/Ca2+ stimulation) states.

Henning Urlaub

Principal Investigator
More subprojects

A4: “Revealing the ultrastructural changes that determine the development of a CNS synapse”

Carolin Wichmann

Z3: “Simple multi-color super-resolution imaging by 10x expansion microscopy”

Silvio Rizzoli

A1: “The ultrastructure of synapses in action”

Benjamin Cooper