State-of-the-art mass spectrometry (MS) makes possible the identification and quantification of cellular proteins, including the detailed analysis of post-translational modifications. MS has recently been combined with chemical cross-linking of proteins, with the purpose of identifying protein–protein contact sites. I now plan to extend cross-linking mass spectrometry to the investigation of protein interactions from native material. I will investigate isolated nerve terminals (synaptosomes) in resting and stimulated (K+/Ca2+ stimulation) states.

Henning Urlaub

Principal Investigator
More subprojects

B3: “Mapping the protein and lipid organization in the plasma membrane of neurons using rapid rupture event imaging”

Andreas Janshoff

B1: “The organization of structural lipids in synaptic membranes”

Nhu Phan

C2: “Dual-purpose optimization theory for the synaptic vesicle cycle”

Fred Wolf