Several techniques used in biomedical research rely on the use of specific affinity probes, such as antibodies. However, antibodies have many problems, due to their large size, polyclonality, bivalency, and the random positioning of the detectable elements. Here we propose to develop and characterize camelid-derived single-domain antibodies (sdAbs or nanobodies) to be used in super-resolution microscopy, cryo-electron tomography (Cryo-ET), and electron microscopy (EM) techniques in SFB1286.

Dr. Felipe Opas

Felipe Opazo

Principal Investigators
More subprojects

B1: “The distribution of structural lipids in synaptic membranes”

Nhu Phan

B3: “Mapping the protein and lipid organization in the plasma membrane of neurons using rapid rupture event imaging”

Andreas Janshoff

B9: “Cytoskeletal alterations contributing to synapto-axonal dysfunction in Parkinson’s disease”

Paul Lingor