Content

Several techniques used in biomedical research rely on the use of specific affinity probes, such as antibodies. However, antibodies have many problems, due to their large size, polyclonality, bivalency, and the random positioning of the detectable elements. Here we propose to develop and characterize camelid-derived single-domain antibodies (sdAbs or nanobodies) to be used in super-resolution microscopy, cryo-electron tomography (Cryo-ET), and electron microscopy (EM) techniques in SFB1286.