We currently lack an overall understanding of the kinetic processes of vesicle-protein interactions. The mobility and diffusivity of proteins is very likely altered by the presence of binding partners such as vesicles, but there is little quantitative information available on this process. To solve this problem, we will combine controlled confining geometries in microfluidic environments with direct in situ single molecule imaging, relying on purified synaptic vesicles and proteins.

Sarah Köster

Principal Investigator

Silvio Rizzoli

Principal Investigator
More subprojects

A4: “Revealing the ultrastructural changes that determine the development of a CNS synapse”

Carolin Wichmann

A3: “A dynamic analysis of the nanoscale organization of the dendritic spine”

Silvio Rizzoli

Associated project: “Modeling of short-term plasticity of nerve-evoked EPSCs at a glutamatergic synapse”

Erwin Neher