During the previous funding period, we verified the hypothesis that synaptic vesicles bind soluble proteins, including exo- and endocytosis cofactors, and maintain them in the synapse. We now plan to follow up on these findings by testing the link between the synaptic vesicle cluster and the cytoskeleton of the presynapse. We will differentiate between two hypotheses of presynaptic organization: 1) the synaptic cytoskeleton serves to organize the vesicle clusters, and 2) alternatively, the vesicles organize the synaptic cytoskeleton. We will use a combination of in vitro imaging and in vitro manipulation tools.

Sarah Köster

Principal Investigator

Silvio Rizzoli

Principal Investigator
More subprojects

B3: “Mapping the protein and lipid organization in the plasma membrane of neurons using rapid rupture event imaging”

Andreas Janshoff

B9: “Cytoskeletal alterations contributing to synapto-axonal dysfunction in Parkinson’s disease”

Paul Lingor

Z3: “Simple multi-color super-resolution imaging by 10x expansion microscopy”

Silvio Rizzoli