Cytoskeletal rearrangements and dynamics orchestrate several processes at the pre- and postsynaptic level. By benefitting from the recent developments of MINFLUX, a nanoscopy technique we invented, which is the first super-resolved optical method with true molecular-size resolution, we will elucidate the exact nanoscale organization of the synaptic actin cytoskeleton. We will shed light on the dynamics and molecular status of actin as a function of synaptic activity and strength by systematically analyzing the abundance and localization of proteins that regulate actin stability and dynamics.

Dr. Elisa D'Este

Elisa D’Este

Principal Investigator

Stefan Hell

Principal Investigator
More subprojects

B9: “Cytoskeletal alterations contributing to synapto-axonal dysfunction in Parkinson’s disease”

Paul Lingor

Z3: “Simple multi-color super-resolution imaging by 10x expansion microscopy”

Silvio Rizzoli

B1: “The distribution of structural lipids in synaptic membranes”

Nhu Phan